expression in Escherichia coli Cyclodextrin glucanotransferase (CGTase) is used for catalytic production of cyclodextrins and various glycosylated products.

Engineering CGTase to improve synthesis of alkyl glycosides. Enzyme synergy for the production of arabinoxylo-oligosaccharides from highly substituted

The CGTase production was further studied with the optimized process parameters on submerged cultivations (SC) and solid-state cultivations (SSC) using soybean industrial fibrous residue (SIFR). The maximum CGTase activity obtained on SC was 1,155 U mL(-1) under aerobic conditions. CGTases are produced by a variety of bacteria, mainly Bacillus species, by submerged culture in complex medium. CGTases differ in the amount and types of CDs produced. In addition, CGTase production is highly dependent on the strain, medium composition and culture conditions.

Cgtase production

Influence of Sodium ion production of CGTase Liquid medium described by Nakamura and Horikoshi (18) was added of 1% Na 2CO 3 to raise the pH to 10. In order to verify the effect of sodium ion on the CGTase production strains were grown in the same medium replacing Na 2CO 3 by NaCl, at pH 7.0. Qualitative analysis of CGTase The CGTase production process consists of a submerged culture fermentation using the recombinant producer organism Bacillus licheniformis strain SJ1608, the stock culture and fermentation culture both being controlled frequently for identity of the organism, absence of contaminating microorganisms, and enzyme yield before harvesting the enzyme. The Bacillus macerans cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) was covalently immobilised on Eupergit C and used in a packed-bed reactor to investigate the continuous production of long-carbohydrate-chain alkyl glycosides from alpha-cyclodextrin (alpha-CD) and n-dodecyl-(1,4)-beta-maltopyranoside (C(12)G(2)beta). The US132 CGTase production monitored after 18 hours of induction showed that the use of M9ZB and 2TY medium increased the production by about 1.1-fold (16.5 U/mL) and 1.3-fold (20 U/mL), respectively, in comparison to that obtained by LB broth. However, the use of M9 medium decreased the production to attain only 8 U/mL. CGTase overexpression enabled a burst of reactive oxygen species production and activated pathogenesis-related gene expression, indicating that the transgenic cotton was better prepared to protect itself from infection.

First starch is liquified either by heat treatment or using α-amylase, then CGTase is added for the enzymatic conversion. CGTases produce mixtures of cyclodextrins, thus the product of the conversion results in a mixture of the three main types of cyclic molecules, in ratios that are strictly dependent on the enzyme used: each CGTase has its

First starch is liquified either by heat treatment or using α-amylase, then CGTase is added for the enzymatic conversion. The industrial production of CGTase was made attractive only when alkaliphilic Bacillus species were introduced as producing organism (23).

Conclusion: The genotoxicity assays and repeated dose toxicity study support the safe use of CGTase in production of alpha-glycosyl isoquercitrin.

To Maximum CGTase production was obtained at 37°C at pH 8. CGTase CGTase producing Bacillus sp. from soil and standardization of its production conditions glycosyltransferase (CGTase) producing strain of newly isolated and mutated Bacillus sp.

and later enzyme characterization. The Bacillus sp. strain was isolated from a Colocacia esculenta rizospheric soil sample and the CGTase production was In view of this, effect of tapioca starch on CGTase production by the alkalophile was evaluated. From our findings, low concentration of tapioca starch (1% w/v) gives higher CGTase production. Illias et al 24 reported maximum CGTase production with 1% tapioca starch as the carbon source for Bacillus sp.
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Cyclodextrins (CD) are cyclic oligosaccharides derived from starch. They are synthesized industrially by cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) [ 1, 2 ]. The smallest naturally occurring CD produced by CGTases is cyclomaltohexaose (CD6) composed of six glucose monomers [ 3 ].

2.3. CGTase cyclizing activity Background. Cyclodextrins (CD) are cyclic oligosaccharides derived from starch. They are synthesized industrially by cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) [ 1, 2 ].
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Cyclodextrin glycosyltransferase (CGTase) catalyzes starch conversion into cyclic or linear oligosaccharides, important industrial products for the complexation of non-polar substances. In this work, conditions to increase CGTase production from Bacillus circulans strain DF 9R were optimized by two systems. On one hand, free cells were grown in batch fermentation experiments to optimize

[10] CGTase production The selected strain was cultivated in flasks containing 200 mL of culture medium and incubated at 37ºC during 18 hours at 200 rpm.